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<title>Journal-of-Protocols-Biological-Methods-ISSUE VOLUME Volume 4 ISSUE Issue 1</title>
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Journal-of-Protocols-Biological-Methods: VOLUME Volume 4 ISSUE Issue 1, Jan-Dec 2024
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<title>Journal-of-Protocols-Biological-Methods-ISSUE VOLUME Volume 4 ISSUE Issue 1</title>
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		<title>Anesthesia-and-Analgesia-Recommended-for-Painful-Procedures-in-Small-Animals</title>
		<pubDate>10-May-2024</pubDate>
<link>http://jpbm.edwiserinternational.com/admin/uploads/IczAGh.pdf</link>
		<author>Amol-W-There</author>
		<comments>{http://www.edwiserinternational.com/contact-us.php}</comments>
		<category>Pharmaceutical Science,Medical Science,Clinical Science</category>
		<description>{<![CDATA[The non-invasive but painful and invasive procedures are frequently performed in experimental animal studies, such as blood withdrawal, route of administration, and surgical procedures. Most of these procedures commonly conducted under anesthesia and/or analgesia and should be complied with the ethical requirements. However, pain is difficult to assess in animals, therefore, it is advisable to provide the analgesics pre-emptively or if painful condition is expected. Preanesthetic like tranquilizer is generally used to reduce the stress in animal and further it also aids in the smooth induction of anesthesia and recovery. In view of this, the objective is to demonstrate and discuss the guidelines of anesthesia and analgesia in small animals, especially mouse and rat with principal focus to ethical purview. These guidelines can be applicable to the animal house facility, pharmacology laboratory, and to a large extent for veterinary care.]]>}</description>
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		<title>A-Review-on-Techniques-of-Blood-Withdrawal-in-Experimental-Small-Animals</title>
		<pubDate>28-May-2024</pubDate>
<link>http://jpbm.edwiserinternational.com/admin/uploads/9vdESc.pdf</link>
		<author>Amol-W-There</author>
		<comments>{http://www.edwiserinternational.com/contact-us.php}</comments>
		<category>Pharmaceutical Science,Medical Science,Clinical Science</category>
		<description>{<![CDATA[In preclinical research, experimental studies in small animals like pharmacokinetics/ toxicokinetics, serum biochemistry and hematology necessitate the biological matrix sampling to estimate drug level, serum enzyme, and blood parameters respectively. The biological matrix is withdrawn from experimental animals by employing the recommended blood collection technique and must be conducted in compliance with the ethical guidelines. It has demanded a minimal painful and least stressful procedures, which can be achieved using inhalant/parenteral anesthesia and/or proper restraint technique. This leads to better experimental outcome. The study personnel conducting blood withdrawal must be well-versed. All these procedures must be approved by IAEC protocol and should include scientist and study personnel names who are involved in animal handling and blood withdrawal. In this review, the different techniques of blood withdrawal from small animals, including collection guidelines, limit volumes and recovery periods, and advantages and disadvantages of sites used for blood collection are demonstrated and discussed.]]>}</description>
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